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mir-seek 🔬

An awesome microRNA-sequencing pipeline
DOI GitHub release Docker Pulls
tests docs GitHub issues GitHub license

This is the home of the pipeline, mir-seek. Its long-term goals: to accurately estimate microRNA expression, to perform quality-control, and to boldly discover known and novel miRNAs like no pipeline before!

Overview

Welcome to mir-seek's documentation! This guide is the main source of documentation for users that are getting started with the miR-sequencing pipeline.

The ./mir-seek pipeline is composed several inter-related sub commands to setup and run the pipeline across different systems. Each of the available sub commands perform different functions:

mir-seek run
Run the mir-seek pipeline with your input files.

mir-seek unlock
Unlocks a previous runs output directory.

mir-seek install
Download remote reference files locally.

mir-seek cache
Cache remote software containers locally.

mir-seek is a comprehensive microRNA-sequencing pipeline. It relies on technologies like Singularity1 to maintain the highest-level of reproducibility. The pipeline consists of a series of data processing and quality-control steps orchestrated by Snakemake2, a flexible and scalable workflow management system, to submit jobs to a cluster.

The pipeline is compatible with data generated from Illumina short-read sequencing technologies. As input, it accepts a set of single-end FastQ files and can be run locally on a compute instance or on-premise using a cluster. A user can define the method or mode of execution. The pipeline can submit jobs to a cluster using a job scheduler like SLURM (more coming soon!). A hybrid approach ensures the pipeline is accessible to all users.

Before getting started, we highly recommend reading through the usage section of each available sub command.

For more information about issues or trouble-shooting a problem, please checkout our FAQ prior to opening an issue on Github.

Contribute

This site is a living document, created for and by members like you. mir-seek is maintained by the members of NCBR and is improved by continous feedback! We encourage you to contribute new content and make improvements to existing content via pull request to our GitHub repository .

Citation

Please note that more citation formats and releases can be found on the mir-seek zenodo page.

If you use this software, please cite it as below:

@software{Kuhn_OpenOmics_mir-seek_2024,
  author       = {Skyler, Kuhn and
                  Gary, Zhang},
  title        = {OpenOmics/mir-seek: v0.3.0},
  month        = sep,
  year         = 2024,
  publisher    = {Zenodo},
  version      = {v0.3.0},
  doi          = {10.5281/zenodo.13834238},
  url          = {https://doi.org/10.5281/zenodo.13834238}
}
Skyler, K., & Gary, Z. (2024). OpenOmics/mir-seek: v0.3.0 (v0.3.0). Zenodo. https://doi.org/10.5281/zenodo.13834238

References

1. Kurtzer GM, Sochat V, Bauer MW (2017). Singularity: Scientific containers for mobility of compute. PLoS ONE 12(5): e0177459.
2. Koster, J. and S. Rahmann (2018). "Snakemake-a scalable bioinformatics workflow engine." Bioinformatics 34(20): 3600.


Last update: 2024-10-01
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